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KMID : 0613820190290020231
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Journal of Life Science
2019 Volume.29 No. 2 p.231 ~ p.238
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In Vitro Antioxidant Activity of Alnus firma Extracts
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Choi Hye-Jung
Joo Woo-Hong
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Abstract
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This study evaluated the antioxidant activity of the extract and fractions of Alnus firma. Alnus firma had the highest total phenolic content (452.80±7.01 μg gallic acid equivalents/mg) in a methanol (MeOH) fraction and the highest total flavonoid content (112.29±11.14 μg rutin equivalents/mg) and antioxidant capacity (936.23±0.07 μg α-tocopherol equivalents/mg) in an ethylacetate (EA) fraction. The antioxidant activities of various solvent extract fractions of Alnus firma were evaluated using various antioxidant assays, including β-carotene?linoleate assay, reducing power assay, 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging assay, metal chelating activity assay, superoxide anion radical scavenging assay, and lipid peroxidation inhibition assay using the ferric thiocyanate method. These activities were compared with those of ascorbic acid, butylated hydroxyanisole (BHA), gallic acid (GA), butylated hydroxytoluene (BHT), and α-tocopherol. First, at a 250 μg/ml concentration, the EA and MeOH fractions of A. firma showed 92.43% and 89.20% DPPH radical scavenging activity, respectively. Second, 50 μg/ml of the EA fraction exhibited 72.49% superoxide anion radical scavenging activity, a little greater than the same dose of GA (60.88%). Finally, 0.5 and 1 mg/ml of the EA fraction showed 73.45% and 73.29% inhibition of peroxidation in the β-carotene?linoleic acid system, respectively. The decreasing order of reducing power was EA fraction > n-butanol (BuOH) fraction > dichloromethane (DCM) fraction > n-hexane (HX) fraction. The results obtained in the present study indicated that Alnus firma can be used as an easily accessible potential source of natural antioxidants.
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KEYWORD
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Alnus firma, antioxidant, antioxidant capacity, flavonoids, reducing power
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